![]() The intensity is plotted as a function of time and the time segments are cut into short time intervals or frames. The orbit in this example is not smooth and only along one position along the cell protrusion. RICS along a line from the nano-scale precise imaging by rapid beam oscillation (nSPIRO) method. In particular we are investigating tumor invasion and executing a strategy to monitor signaling for cells migrating in 3D matrices and in tissues. Using the raster image correlation spectroscopy (RICS) method and the Number and Molecular Brightness (N&B) analysis we have revealed the dynamic assembly and disassembly of focal adhesions as well as determined the aggregation and stoichiometry of protein interaction. We are using the cutting edge biophysical tools to monitor molecular interactions in live cells and how their activity in spatial locations mediates cell migration. 1) quantifying spatial and temporal Rac 1A and Rho-A activation during cell migration,Ģ) characterizing metabolic alterations in cancer and degenerative diseased cells and tissues,ģ) correlating mitochondrial transport with cancer cell invasion,Ĥ) fine tuning cancer metabolism in response to matrix stiffnessĬell migration is vital for embryonic development, wound healing, and cancer metastasis.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |